Hi, need to submit a 4500 words essay on the topic Project: Analysis for the expression levels of RHoB protein in breast cancer cell lines.
This was done with emphasis on RHoB protein. RhoB protein is not important in the development but it impacts the growth and adhesion factors of the transformed cells. The RNA was isolated from the 3 cell. The cDNA library was then constructed using the isolated RNA as the template strand. This step was after isolation and purification. The samples were later amplified using PCR and analyzed by gel electrophoresis and imaging. GAPDH primers were used in each cell lines in order to compare the results obtained. Positive control of GAPDH was also used to eliminate any extraneous variables. It is well known that RNA/mRNA is directly proportional to the proteins being expressed in the cell. In this case we measured the level of RNA to help us determine the level of expression of the protein of interest which is RhoB protein in the three cell lines that include MCF10 A, MCF7 and MDA – MB 231. According to the results, it is clearly evident that the three cell lines had different levels of the RhoB proteins that were expressed
RhoB can be defined as a quick early reaction gene quickly inducible by numerous jolts including genotoxic anxiety, cytokines and development variables (Gerald, 2013). In contrast, despite RhoB being related to vast family comprising of GTPases (RhoA/Rac1/Cdc42), RhoB is uniquely located in the cytoplasm (Huang et al., 2007). In relation to this, it is most precisely found on the endosome. RhoB is linked with regulation of vesicles and also in trafficking of growth factor receptors (Gerald, 2013). The function of the RhoB protein is to mediate apoptosis in cells that have been transformed neo-plastically after DNA damage (Fritz and Kaina 1997). RhoB protein is not important in the development but it impacts the growth and adhesion factors of the transformed cells. It usually plays a negative role in tumor genesis because when it is deleted, it contributes to the formation of tumors cells.